Tuberculosis
- Identification of protective postexposure mycobacterial vaccine antigens using an immunosuppression-based reactivation model in the zebrafish
Summary: We characterize the reactivation of a latent mycobacterial infection and identify DNA-based vaccine candidates against the reactivation of tuberculosis using the adult zebrafish Mycobacterium marinum infection model.
- Priming of innate antimycobacterial immunity by heat-killed Listeria monocytogenes induces sterilizing response in the adult zebrafish tuberculosis model
Summary: Heat-killed Listeria monocytogenes induces immune responses that lead to increased clearance of mycobacterial infection in the adult zebrafish tuberculosis model via innate immune mechanisms.
- Microglia activation in a pediatric rabbit model of tuberculous meningitis
Summary: A clinically relevant novel rabbit model provides robust microglial activation and neurological deficits as seen in children with pediatric central nervous system tuberculosis.
- Mouse model of pulmonary cavitary tuberculosis and expression of matrix metalloproteinase-9
Summary: We demonstrate for the first time a murine model that consistently produces pulmonary cavitary TB and provides a tractable and economical new tool for better understanding of cavitation.
- CLARITY and PACT-based imaging of adult zebrafish and mouse for whole-animal analysis of infections
Summary: The authors apply the CLARITY and PACT techniques to image infectious structures located deep within whole adult zebrafish and mouse organs, enabling visualization of mycobacterial granulomas and host response without sectioning.
- Lung necrosis and neutrophils reflect common pathways of susceptibility to Mycobacterium tuberculosis in genetically diverse, immune-competent mice
Summary: Molecular biomarkers of tuberculosis are identified and used to classify disease status of Diversity Outbred mice that have been infected with Mycobacterium tuberculosis.
- Presence of multiple lesion types with vastly different microenvironments in C3HeB/FeJ mice following aerosol infection with Mycobacterium tuberculosis
Summary: C3HeB/FeJ mice develop three morphologically distinct lesion types, which differ with respect to bacterial load, cellular composition and degree of immunopathology following low-dose aerosol infection with Mycobacterium tuberculosis.
- Heterogeneous disease progression and treatment response in a C3HeB/FeJ mouse model of tuberculosis
Summary: C3HeB/FeJ mice develop a wide range of lesion types that alter drug response in a way that might better inform tuberculosis drug development.