CRISPR/Cas9
- CRISPR/Cas9-mediated genome editing in nonhuman primates
Summary: This Review discusses the history and development of genome editing in non-human primates, as well as the challenges and prospects facing this technology now and in the future.
- Experimental models and tools to tackle glioblastoma
Summary: This Review discusses preclinical modelling of glioblastoma multiforme to understand its biology and develop therapies, with a focus on mammalian model systems.
- Modeling epigenetic modifications in renal development and disease with organoids and genome editing
Summary: In this Review, we provide an overview on how epigenetic processes are altered in kidney development and disease, and discuss how CRISPR-modified kidney organoids can help us to understand the function of epigenetic marks.
- CRISPR/Cas9-mediated homology-directed repair by ssODNs in zebrafish induces complex mutational patterns resulting from genomic integration of repair-template fragments
Summary: NGS-based analysis reveals that CRISPR/Cas9-induced double-strand-break repair using single-stranded repair templates is error prone in zebrafish, resulting in complex patterns of integrated repair-template fragments.
- Effective CRISPR/Cas9-based nucleotide editing in zebrafish to model human genetic cardiovascular disorders
Summary: By using a single-stranded DNA oligonucleotide template in combination with CRISPR/Cas9 in zebrafish, the authors achieved effective germline-transmissible introduction of patient-specific single-nucleotide changes related to cardiovascular disease.
- Apolipoprotein E deficiency accelerates atherosclerosis development in miniature pigs
Editor's choice: ApoE knockout pigs displayed severe hypercholesterolemia and spontaneously developed human-like atherosclerotic lesions in the aorta and coronary arteries within 6 months of feeding on a high-fat and high-cholesterol diet.
- A severe atherosclerosis mouse model on the resistant NOD background
Summary: Double knockout of Apoe and Ldlr on the highly atherosclerosis-resistant NOD mouse background results in severe atherosclerosis, which paves the way for the study of severe atherosclerosis in the setting of autoimmunity.
- RNaseT2 knockout rats exhibit hippocampal neuropathology and deficits in memory
Summary: The authors characterize a rodent model of RNaseT2 deficiency, which offers insight into the susceptibility of the hippocampus to early inflammation caused by lysosome impairment due to loss of RNaseT2 function.
- Truncated C-terminus of fibrillin-1 induces Marfanoid-progeroid-lipodystrophy (MPL) syndrome in rabbit
Summary: A novel genetically engineered rabbit model of MPL syndrome, generated by CRISPR/Cas9-mediated mutation of FBN1, mimics the histopathological changes and functional defects of MPL syndrome seen in the clinic.