PT  - JOURNAL ARTICLE
AU  - Lee, Eunjo
AU  - Kim, Namkyung
AU  - Kang, Jinjoo
AU  - Yoon, Sangwon
AU  - Lee, Hae-Ahm
AU  - Jung, Hanna
AU  - Kim, Sang-Hyun
AU  - Kim, Inkyeom
TI  - Activated pathogenic Th17 lymphocytes induce hypertension following high-fructose intake in Dahl salt-sensitive but not Dahl salt-resistant rats
AID  - 10.1242/dmm.044107
DP  - 2020 May 01
TA  - Disease Models & Mechanisms
PG  - dmm044107
VI  - 13
IP  - 5
4099  - http://dmm.biologists.org/content/13/5/dmm044107.short
4100  - http://dmm.biologists.org/content/13/5/dmm044107.full
SO  - Dis Models Mech2020 May 01; 13
AB  - High-salt intake and high-fructose intake are risk factors for hypertension via oxidative stress and inflammation. T helper (Th)17 lymphocytes play an important role in the development of hypertension. Here, we tested the hypothesis that activation of pathogenic Th17 lymphocytes induces hypertension after high-fructose intake in Dahl salt-sensitive (SS) but not Dahl salt-resistant (SR) rats. Eight-week-old male SS and SR rats were offered 20% fructose solution or tap water only for 4 weeks. Systolic blood pressure was measured by the tail-cuff method. T lymphocyte [Th17 and T regulatory (Treg)] profiling was determined via flow cytometry. The expression of Th17-related (IL-17A, IL-17RA, IL-23R and RORγt) and Treg-related (IL-10, CD25, FOXP3 and TGFβ) factors were measured via ELISA or qRT-PCR. Th17 lymphocytes isolated from high-fructose-fed SS rats were intraperitoneally injected into recipient SS and SR rats, and recombinant IL-23 protein was subcutaneously injected into SS and SR rats to induce hypertension.High-fructose intake induced hypertension via the activation of pathogenic Th17 lymphocytes in SS but not SR rats. Injection of activated Th17 lymphocytes isolated from fructose-fed SS rats induced hypertension via increase of serum IL-17A only in recipient SS rats. In addition, injection of IL-23 induced hypertension via activation of pathogenic Th17 lymphocytes only in SS rats.Thus, activation of pathogenic Th17 lymphocytes induces hypertension after high-fructose intake in SS but not SR rats. These results indicate that immunologic tolerance plays an important role in protection against hypertension in SR rats.