EDITORIAL
- Drug screening using model systems: some basics
Summary: The author provides some tips on what to consider before you begin drug screening.
REVIEW
- Preclinical models for obesity research
Summary: We review genetic models of obesity, their optogenetic and chemogenetic successors, and the use of dietary manipulations and meal-feeding regimes.
RESEARCH ARTICLES
- Development of a subset of forelimb muscles and their attachment sites requires the ulnar-mammary syndrome gene Tbx3
Summary: The ulnar-mammary syndrome (UMS) gene, Tbx3, is required for development of posterior forelimb bones, muscles and their attachment sites. This broadens the UMS phenotype and suggests a new muscle-specification model.
- Insulin receptor isoform A ameliorates long-term glucose intolerance in diabetic mice
Summary: The specific hepatic expression of insulin receptor isoform A, but not isoform B, is able to revert, in the long term, the global glucose intolerance observed in diabetic mice.
- Upregulation of the Nr2f1-A830082K12Rik gene pair in murine neural crest cells results in a complex phenotype reminiscent of Waardenburg syndrome type 4
Summary: A forward genetic screen approach unveils a new mechanism, involving Nr2f1 and/or A830082K12Rik overexpression in neural crest cells, that could promote development of Waardenburg syndrome and Hirschsprung disease.
- Immortalized Parkinson's disease lymphocytes have enhanced mitochondrial respiratory activity
Editors' choice: Cultured Parkinson's disease lymphocytes are metabolically hyperactive, suggesting a new understanding of the underlying cytopathology and biomarkers for this and potentially other neurodegenerative diseases.
- Gli2 gene-environment interactions contribute to the etiological complexity of holoprosencephaly: evidence from a mouse model
Summary: This work illustrates how a specific genetic predisposition in combination with exposure to an environmental factor can result in a severe birth defect, providing a new opportunity to develop prevention strategies.
- Restoration of mutant bestrophin-1 expression, localisation and function in a polarised epithelial cell model
Summary: Chemical chaperone 4PBA fully restores Cl− conductance activity for mutant bestrophin-1 proteins associated with inherited retinal dystrophy, autosomal recessive bestrophinopathy.
- Cartilage damage and bone erosion are more prominent determinants of functional impairment in longstanding experimental arthritis than synovial inflammation
Summary: Gait-profile assessment is an objective quantitative measurement to monitor functional impairment in arthritis models. Longstanding functional impairment correlates with inflammation-driven structural damage.
- Pharmacological treatment and BBB-targeted genetic therapy for MCT8-dependent hypomyelination in zebrafish
Summary: Expression of thyroid transporter specifically in the blood–brain barrier of zebrafish, and pharmacological treatments with thyroid hormone analogs and clemastine, rescue myelin deficiencies.
- HSV presence in brains of individuals without dementia: the TASTY brain series
Summary: We assessed the presence of HSV types 1 and 2 in the brain tissue of a large non-institutionalised autopsy cohort, providing evidence of asymptomatic access of HSV to the brain.
- Photoperiod induced obesity in the Brandt's vole (Lasiopodomys brandtii): a model of ‘healthy obesity’?
Summary: Obesity induced by long-day photoperiod in voles is not accompanied by impaired glucose tolerance or insulin sensitivity and might be a useful model of ‘healthy obesity’.
- Function of Ltbp-4L and fibulin-4 in survival and elastogenesis in mice
Summary: The interaction of the long form of latent-transforming growth factor beta-binding protein 4 and fibulin-4 is essential for survival as well as for the formation of elastic fibers.
- Acute and long-term outcomes in a Drosophila melanogaster model of classic galactosemia occur independently of galactose-1-phosphate accumulation
Summary: In a GALT-deficient Drosophila model of classic galactosemia, Gal-1P accumulation is not required for compromised larval survival following galactose exposure or adult movement and fecundity phenotypes.
- Inhibition of vascular endothelial growth factor signaling facilitates liver repair from acute ethanol-induced injury in zebrafish
Summary: Inhibition of VEGF signaling enhances recovery from acute ethanol-induced injury in the livers of zebrafish larvae, by directly suppressing fibrogenesis and angiogenesis, and indirectly ameliorating hepatic steatosis.
RESOURCE ARTICLES
- High- and ultrahigh-field magnetic resonance imaging of naïve, injured and scarred vocal fold mucosae in rats
Summary: Magnetic resonance imaging is shown to allow the nondestructive assessment of acute injury and scar formation in vocal fold mucosa, as demonstrated ex vivo using a preclinical rat model.
- An in vitro model of murine middle ear epithelium
Summary: Development and systematic characterisation of an in vitro otopathogenic infection model of the murine middle ear epithelium as a tool to better understand the complex pathophysiology of Otitis media.
This issue
Other journals from The Company of Biologists
DMM and COVID-19
We are aware that the COVID-19 pandemic is having an unprecedented impact on researchers worldwide. The Editors of all The Company of Biologists’ journals have been considering ways in which we can alleviate concerns that members of our community may have around publishing activities during this time. Read about the actions we are taking at this time.
Please don’t hesitate to contact the Editorial Office if you have any questions or concerns.
Alessandro Bailetti is awarded the DMM Outstanding Paper Prize 2019
The annual prize is awarded to the first author of the paper that is judged by the journal’s editors to be the most outstanding contribution to the journal that year. Alessandro Bailetti's winning paper presents results that indicate a possible connection between E(Pc) and the Tip60 complex, and leukemia. Find out more about Alessandro and read the winning paper.
Find DMM online
We may not be meeting you in person this year, but we’re always here to chat about DMM and The Company of Biologists. Join the conversation over on Twitter, email us or catch Scientific Editor Julija Hmeljak at these upcoming virtual meetings:
MRC Human Genetics Unit (Virtual) Symposium: Aberrant Cell State Transitions in Human Disease
23-24 September
Genomic Imprinting – from Biology to Disease
28 September – 1 October
ZDMS Virtual webinar series
Throughout September – December
Interview - Pratibha Singh
First author Pratibha Singh discusses her intestinal injury model in mouse neonatal pups, the most significant challenge impacting her field of research and shares her thoughts on how to better support early-career researchers.
Call for papers - The RAS Pathway: Diseases, Therapeutics and Beyond
DMM invites you to submit original research for a special issue focused on targeting the RAS pathway. The submission deadline is 1 March 2021. Find out more and showcase your breakthrough research in this special collection.
It’s a time of turmoil for young scientists
“My students are my priority – they are on the brink of their careers.”
Dr Jodie Rummer (ARC Centre of Excellence for Coral Reef Studies at James Cook University) talks about the importance of her role as a supervisor in supporting graduate students and post docs financially, intellectually and emotionally during this period of uncertainty.