EDITORIAL
- Drug screening using model systems: some basics
Summary: The author provides some tips on what to consider before you begin drug screening.
REVIEW
- Preclinical models for obesity research
Summary: We review genetic models of obesity, their optogenetic and chemogenetic successors, and the use of dietary manipulations and meal-feeding regimes.
RESEARCH ARTICLES
- Development of a subset of forelimb muscles and their attachment sites requires the ulnar-mammary syndrome gene Tbx3
Summary: The ulnar-mammary syndrome (UMS) gene, Tbx3, is required for development of posterior forelimb bones, muscles and their attachment sites. This broadens the UMS phenotype and suggests a new muscle-specification model.
- Insulin receptor isoform A ameliorates long-term glucose intolerance in diabetic mice
Summary: The specific hepatic expression of insulin receptor isoform A, but not isoform B, is able to revert, in the long term, the global glucose intolerance observed in diabetic mice.
- Upregulation of the Nr2f1-A830082K12Rik gene pair in murine neural crest cells results in a complex phenotype reminiscent of Waardenburg syndrome type 4
Summary: A forward genetic screen approach unveils a new mechanism, involving Nr2f1 and/or A830082K12Rik overexpression in neural crest cells, that could promote development of Waardenburg syndrome and Hirschsprung disease.
- Immortalized Parkinson's disease lymphocytes have enhanced mitochondrial respiratory activity
Editors' choice: Cultured Parkinson's disease lymphocytes are metabolically hyperactive, suggesting a new understanding of the underlying cytopathology and biomarkers for this and potentially other neurodegenerative diseases.
- Gli2 gene-environment interactions contribute to the etiological complexity of holoprosencephaly: evidence from a mouse model
Summary: This work illustrates how a specific genetic predisposition in combination with exposure to an environmental factor can result in a severe birth defect, providing a new opportunity to develop prevention strategies.
- Restoration of mutant bestrophin-1 expression, localisation and function in a polarised epithelial cell model
Summary: Chemical chaperone 4PBA fully restores Cl− conductance activity for mutant bestrophin-1 proteins associated with inherited retinal dystrophy, autosomal recessive bestrophinopathy.
- Cartilage damage and bone erosion are more prominent determinants of functional impairment in longstanding experimental arthritis than synovial inflammation
Summary: Gait-profile assessment is an objective quantitative measurement to monitor functional impairment in arthritis models. Longstanding functional impairment correlates with inflammation-driven structural damage.
- Pharmacological treatment and BBB-targeted genetic therapy for MCT8-dependent hypomyelination in zebrafish
Summary: Expression of thyroid transporter specifically in the blood–brain barrier of zebrafish, and pharmacological treatments with thyroid hormone analogs and clemastine, rescue myelin deficiencies.
- HSV presence in brains of individuals without dementia: the TASTY brain series
Summary: We assessed the presence of HSV types 1 and 2 in the brain tissue of a large non-institutionalised autopsy cohort, providing evidence of asymptomatic access of HSV to the brain.
- Photoperiod induced obesity in the Brandt's vole (Lasiopodomys brandtii): a model of ‘healthy obesity’?
Summary: Obesity induced by long-day photoperiod in voles is not accompanied by impaired glucose tolerance or insulin sensitivity and might be a useful model of ‘healthy obesity’.
- Function of Ltbp-4L and fibulin-4 in survival and elastogenesis in mice
Summary: The interaction of the long form of latent-transforming growth factor beta-binding protein 4 and fibulin-4 is essential for survival as well as for the formation of elastic fibers.
- Acute and long-term outcomes in a Drosophila melanogaster model of classic galactosemia occur independently of galactose-1-phosphate accumulation
Summary: In a GALT-deficient Drosophila model of classic galactosemia, Gal-1P accumulation is not required for compromised larval survival following galactose exposure or adult movement and fecundity phenotypes.
- Inhibition of vascular endothelial growth factor signaling facilitates liver repair from acute ethanol-induced injury in zebrafish
Summary: Inhibition of VEGF signaling enhances recovery from acute ethanol-induced injury in the livers of zebrafish larvae, by directly suppressing fibrogenesis and angiogenesis, and indirectly ameliorating hepatic steatosis.
RESOURCE ARTICLES
- High- and ultrahigh-field magnetic resonance imaging of naïve, injured and scarred vocal fold mucosae in rats
Summary: Magnetic resonance imaging is shown to allow the nondestructive assessment of acute injury and scar formation in vocal fold mucosa, as demonstrated ex vivo using a preclinical rat model.
- An in vitro model of murine middle ear epithelium
Summary: Development and systematic characterisation of an in vitro otopathogenic infection model of the murine middle ear epithelium as a tool to better understand the complex pathophysiology of Otitis media.
This issue
Other journals from The Company of Biologists
DMM and COVID-19
We are aware that the COVID-19 pandemic is having an unprecedented impact on researchers worldwide. The Editors of all The Company of Biologists’ journals have been considering ways in which we can alleviate concerns that members of our community may have around publishing activities during this time. Read about the actions we are taking at this time.
Please don’t hesitate to contact the Editorial Office if you have any questions or concerns.
A Model for Life – Elizabeth McNally and Louis Kunkel
Human geneticist and cardiologist Elizabeth McNally discusses her first experiences in science and the effort we all need to make to facilitate diversity in science, while Louis Kunkel tells us about talks about his discovery of dystrophin.
On the cover of our special issue, Demonbreun et al. present muscle fiber typing from a newly created mouse model of limb-girdle muscular dystrophy generated by gene editing.
Non-canonical autophagy in innate immune defence in vivo
preLighter Kirsty Hooper, The Babraham Institute, Cambridge, UK, discusses a preprint showing that loss of non-canonical autophagy in mice increases sensitivity to influenza A virus infection.
The Biology Open transfer option
DMM authors can transfer their submission to our sister journal Biology Open with just one click. An online Open Access journal, BiO also welcomes direct submissions and focuses on the swift publication of high-quality, sound research. Recent disease modelling highlights in BiO include work by Liu et al. investigating transgenic replenishment strategies targeting myocardial and non-myocardial tissues in the zebrafish. Find out more about how to transfer.
Editor’s choice
Highlighted by the Guest editors of our special issue, Grunwald and colleagues use zebrafish to investigate interactions among ryanodine receptor isotypes in muscle development and function, while Claire Wood et al. tests the mdx:Cmah−/− mouse to investigate growth and skeletal development in Duchenne muscular dystrophy.