SUMMARY
Although it is known that tumor necrosis factor receptor (TNFR) signaling plays a crucial role in vascular integrity and homeostasis, the contribution of each receptor to these processes and the signaling pathway involved are still largely unknown. Here, we show that targeted gene knockdown of TNFRSF1B in zebrafish embryos results in the induction of a caspase-8, caspase-2 and P53-dependent apoptotic program in endothelial cells that bypasses caspase-3. Furthermore, the simultaneous depletion of TNFRSF1A or the activation of NF-κB rescue endothelial cell apoptosis, indicating that a signaling balance between both TNFRs is required for endothelial cell integrity. In endothelial cells, TNFRSF1A signals apoptosis through caspase-8, whereas TNFRSF1B signals survival via NF-κB. Similarly, TNFα promotes the apoptosis of human endothelial cells through TNFRSF1A and triggers caspase-2 and P53 activation. We have identified an evolutionarily conserved apoptotic pathway involved in vascular homeostasis that provides new therapeutic targets for the control of inflammation- and tumor-driven angiogenesis.
Footnotes
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COMPETING INTERESTS: The authors declare that they do not have any competing or financial interests.
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AUTHOR CONTRIBUTIONS: F.J.R., R.E. and V.M. designed the research; F.J.R., R.E., S.C., M.P.S., J.M.G.-R., N.M. and F.A.-P., performed research; F.J.R., R.E., S.C., M.P.S., J.M.G.-R., N.M., F.A.-P., M.L.C., J.M. and V.M. analyzed data; and F.J.R. and V.M. wrote the paper with contributions from other authors.
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SUPPLEMENTARY MATERIAL: Supplementary material for this article is available at http://dmm.biologists.Org/lookup/suppl/doi:10.1242/dmm.010249/-/DC1
- Received May 25, 2012.
- Accepted August 18, 2012.
- © 2013. Published by The Company of Biologists Ltd
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.
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