Subject collection: Zebrafish as a Disease Model
- Targeted knockout of GABA-A receptor gamma 2 subunit provokes transient light-induced reflex seizures in zebrafish larvae
Summary: The authors present a novel in vivo genetic model of idiopathic epilepsy in zebrafish (gabrg2−/−) to aid the study of ictogenesis and provide a convenient genetic tool for drug screening.
- Deep learning enables automated volumetric assessments of cardiac function in zebrafish
Summary: The authors present CFIN, a deep learning-based image-analysis platform to automatically analyze dynamic light-sheet fluorescence microscopy images and determine volumetric indices of cardiac function in embryonic zebrafish.
- Reversion of tumor hepatocytes to normal hepatocytes during liver tumor regression in an oncogene-expressing transgenic zebrafish model
Summary: In an oncogene-addicted liver cancer zebrafish model, transformed cancer hepatocytes could be reverted to normal hepatocytes, as shown by Cre-loxP-mediated cell-lineage tracing.
- Haploinsufficiency of mechanistic target of rapamycin ameliorates bag3 cardiomyopathy in adult zebrafish
Summary: This study shows that adult bag3 knockout mutant zebrafish can be used as a model for DCM, and haploinsufficiency of mTOR is cardioprotective.
- Sodium nitroprusside prevents the detrimental effects of glucose on the neurovascular unit and behaviour in zebrafish
Summary: Diabetes is associated with vascular and neurological impairments. The authors show that an NO donor ameliorates the glucose-exposure-induced dysfunction in the tectal neurovascular unit and whole-organism behaviour.
- Modeling hematopoietic disorders in zebrafish
Summary: This At A Glance article and poster summarize the last 20 years of research in zebrafish models for hematopoietic disorders, highlighting how these models were created and are being applied for translational research.
- Clinical pathologies of bone fracture modelled in zebrafish
Summary: The effect of osteogenesis imperfecta, bisphosphonate treatment and bacterial infection on phases of bone fracture repair are determined using a zebrafish fracture model.
- Zebrafish modeling of intestinal injury, bacterial exposures and medications defines epithelial in vivo responses relevant to human inflammatory bowel disease
Summary: As IBD attempts to enter the era of precision medicine, our models provide an opportunity for the rapid and sophisticated determination of time course, cellular contributions and mechanisms of new drugs.
- The zebrafish subcortical social brain as a model for studying social behavior disorders
Summary: The zebrafish has become an increasingly attractive model for studying social behavior disorders. This Review summarizes relevant methods, established social deficit models, emerging technologies and the anatomical basis of sociality.
- Active receptor tyrosine kinases, but not Brachyury, are sufficient to trigger chordoma in zebrafish
Summary: An injection-based chordoma model in zebrafish shows that the hypothesized chordoma oncogene brachyury is insufficient, whereas EGFR and VEGFR2 are sufficient, to trigger notochord hyperplasia in our model.