Subject collection: Organoids
- Inhibition of Alk signaling promotes the induction of human salivary-gland-derived organoids
Summary: Human salivary-gland-derived organoids can be used for in vitro analyses of the morphological and functional changes associated with salivary gland diseases and dysfunctions.
- Neuromuscular disease modeling on a chip
Summary: Modeling neuromuscular diseases is challenging due to their complex etiology and pathophysiology. Here, we review the cell sources and tissue-engineering procedures that are being integrated as emerging neuromuscular disease models.
- Human embryoid bodies as a 3D tissue model of the extracellular matrix and α-dystroglycanopathies
Summary: Human stem cell culture methods enable the generation of extracellular matrix-containing tissue to study patient-specific pathology and experimental treatment of the α-dystroglycanopathies.
- Precision-based modeling approaches for necrotizing enterocolitis
Summary: Much of our understanding of necrotizing enterocolitis (NEC) pathogenesis has been achieved through animal models. Here, we discuss the development of advanced precision-based models to improve outcomes for patients with NEC.
- Disease modelling in human organoids
Summary: Human organoids are important tools for modelling disease. This At a Glance article summarises the current organoid models of several human diseases, and discusses future prospects for these technologies.
- Integrative analysis of Paneth cell proteomic and transcriptomic data from intestinal organoids reveals functional processes dependent on autophagy
Editor's choice: Using an integrative approach encompassing intestinal organoid culture, proteomics, transcriptomics and protein-protein interaction networks, we list Paneth cell functions dependent on autophagy.
- Modeling epigenetic modifications in renal development and disease with organoids and genome editing
Summary: In this Review, we provide an overview on how epigenetic processes are altered in kidney development and disease, and discuss how CRISPR-modified kidney organoids can help us to understand the function of epigenetic marks.
- Modeling the effect of ascites-induced compression on ovarian cancer multicellular aggregates
Summary: Development of new model systems with which to evaluate cellular responses to compression enables experimental modeling of the intraperitoneal force environment of ovarian cancer patients with tense ascites.
- Metastasis in context: modeling the tumor microenvironment with cancer-on-a-chip approaches
Summary: This Review evaluates the recent contributions of cancer-on-a-chip models to our understanding of the tumor microenvironment and its role in the onset of metastasis. The authors also provide an outlook for future applications of this emerging technology.
- Gastroenteropancreatic neuroendocrine neoplasms: genes, therapies and models
Summary: Gastroenteropancreatic neuroendocrine neoplasms have been an understudied entity with limited treatment options. We discuss the disease models, including emerging organoids, that may provide biological and therapeutic insight into these diseases.