Subject collection: Metabolic Disorders
- Sensory neuron cultures derived from adult db/db mice as a simplified model to study type-2 diabetes-associated axonal regeneration defects
Summary: Morphological characterization of a model for evaluating neuritic regeneration in vitro in dorsal root ganglion primary neurons derived from type-2 diabetic mice with an advanced stage of diabetic neuropathy.
- 3D quantification of changes in pancreatic islets in mouse models of diabetes type I and II
Summary: We report a 3D quantitative imaging platform (using light-sheet fluorescence microscopy) that can reveal changes in beta cell volume and proliferation, and leukocyte infiltration in pancreata in mouse models of diabetes.
- A comprehensive study of phospholipid fatty acid rearrangements in metabolic syndrome: correlations with organ dysfunction
Summary: In rats, a high-fat diet induces the selective distribution of fatty acids within phosphatidylcholine in the liver and muscles, in a manner that correlates with organ dysfunction. The cardiovascular system appears to be protected under these conditions.
- Novel defatting strategies reduce lipid accumulation in primary human culture models of liver steatosis
Summary: This study describes a defatting cocktail that has been proven to function in three relevant steatotic human culture models without cytotoxicity, and which could be employed in the reduction of steatosis in donor livers during liver transplantation.
- Familial hypercholesterolemia class II low-density lipoprotein receptor response to statin treatment
Editor's choice: Statin-induced upregulation of class II mutant LDLR does not cause acute endoplasmic reticulum (ER) stress and unfolded protein response induction, in spite of misfolded LDLR protein accumulation in the ER.
- Integrated lipidomic and transcriptomic analyses identify altered nerve triglycerides in mouse models of prediabetes and type 2 diabetes
Summary: Mouse models of prediabetes and type 2 diabetes that develop peripheral neuropathy display increased levels of nerve triglycerides, which return to normal upon dietary reversal, suggesting that altered lipids are involved in disease.
- Cell-specific and athero-protective roles for RIPK3 in a murine model of atherosclerosis
Summary: This study reports that RIPK3 in macrophages and endothelial cells protects against atherosclerosis progression in mice, providing novel information about unexpected roles for RIPK3 in an inflammatory vascular disease.
- Ablation of Bscl2/seipin in hepatocytes does not cause metabolic dysfunction in congenital generalised lipodystrophy
Editor's choice: Hepatic Bscl2 ablation in adipose tissue-specific Bscl2 knockout mice does not cause metabolic dysfunction. CRISPR/Cas9 genome editing reveals that seipin does not play a cell-autonomous role in regulating hepatocyte lipid accumulation.
- Sodium nitroprusside prevents the detrimental effects of glucose on the neurovascular unit and behaviour in zebrafish
Summary: Diabetes is associated with vascular and neurological impairments. The authors show that an NO donor ameliorates the glucose-exposure-induced dysfunction in the tectal neurovascular unit and whole-organism behaviour.
- A new mouse model of GLUT1 deficiency syndrome exhibits abnormal sleep-wake patterns and alterations of glucose kinetics in the brain
Summary: New phenotypes are revealed by a GLUT1 deficiency mutant mouse model carrying a missense mutation in Glut1.