The Wistar Kyoto rat (WKY) and the Spontaneously Hypertensive Rat (SHR) inbred strains are well-established models for human crescentic glomerulonephritis (CRGN) and metabolic syndrome respectively. Novel transgenic (Tg) strains add research opportunities and increase scientific value to well-established rat models. We have created two novel Tg strains using Sleeping Beauty transposon germline transgenesis, ubiquitously expressing green fluorescent protein (GFP) under the rat elongation factor 1 alpha (rEF1a) promoter on the WKY and SHR genetic backgrounds. The Sleeping Beauty system functioned with high transgenesis efficiency in our hands: 75% of the new rats born after embryo microinjections were transgene positive. By ligation-mediated PCR, we located the genome integration sites, confirming no exonic disruption and defining single or low copy number of the transgenes in the new WKY-GFP and SHR-GFP Tg lines. We report GFP bright expression in embryos, tissues and organs in both lines and show preliminary in vitro and in vivo imaging data that show the utility of the new GFP-expressing lines for adoptive transfer, transplantation and fate mapping studies of CRGN, metabolic syndrome and other traits for which these strains have been extensively studied over the past four decades.
- Received November 25, 2015.
- Accepted January 13, 2016.
- © 2016. Published by The Company of Biologists Ltd
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