Myopia is a huge public health problem worldwide reaching the highest incidence in Asia. Identification of susceptible genes is critical for understanding the biological basis of myopia. In this paper, we have identified and characterized a functional myopia-associated gene using a specific mouse-knock out model. Mice lacking muscarinic cholinergic receptor gene (M2) were less susceptible to lens-induced myopia compared to wild type mice, which showed significantly increased axial length and vitreous chamber depth when undergoing experimental induction of myopia. The key findings of this present study is that M2 mutant mice sclera has high expression of collagen type I and low level of collagen type V than WT and other muscarinic sub types mutant mice, and therefore M2 mutant mice were resistant to develop experimental myopia. Pharmacological blockade of M2 muscarinic receptor proteins retarded myopia progression in the mouse. These results suggest for the first time a role of M2 in growth related changes in extra cellular matrix genes during myopia development in a mammalian model. M2 receptor antagonists may thus provide a targeted therapeutic approach to the management of this refractive error.
- Received September 27, 2012.
- Accepted April 29, 2013.
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