Subject collection: Zebrafish as a Disease Model
- ptf1a+, ela3l− cells are developmentally maintained progenitors for exocrine regeneration following extreme loss of acinar cells in zebrafish larvae
Summary: New transgenic models for exocrine pancreas regeneration in zebrafish reveal a novel potential exocrine progenitor as the source for a two-phase regeneration process after complete acinar cell loss.
- Miconazole protects blood vessels from MMP9-dependent rupture and hemorrhage
Summary: A phenotype-based chemical screen in zebrafish identifies miconazole as a novel hemorrhagic suppressor. Miconazole inhibits vessel rupture and hemorrhages by decreasing pErk and MMP9 in zebrafish and rats.
- A novel brain tumour model in zebrafish reveals the role of YAP activation in MAPK- and PI3K-induced malignant growth
Editors' choice: A new brain tumour model in zebrafish allows simultaneous analysis of malignant tumours and heterotopias, suggesting that both lesions originate from similar events, with Yap1 as a driving force in tumour development.
- Standardized echocardiographic assessment of cardiac function in normal adult zebrafish and heart disease models
Summary: Standardization of zebrafish echocardiography provides insights into cardiac physiology in normal and diseased states, with application for functional studies in zebrafish models of heart disease.
- Loss of vhl in the zebrafish pronephros recapitulates early stages of human clear cell renal cell carcinoma
Summary: Zebrafish with an inactivating mutation in the vhl gene can be used as a model of early stage clear cell renal cell carcinoma, with applications for genetic studies and drug screens.
- Telomeres in aging and disease: lessons from zebrafish
Summary: In this Review, the authors explore the advantages of using zebrafish in telomere research and discuss the primary discoveries made in this model that have contributed to expanding our knowledge of how telomere attrition contributes to cellular senescence, organ dysfunction and disease.
- Long-term drug administration in the adult zebrafish using oral gavage for cancer preclinical studies
Summary: We have developed the first long-term drug delivery system in the adult zebrafish using oral gavage, and offer a foundation for future preclinical studies of cancer therapeutics in adult zebrafish.
- Modeling human diseases: an education in interactions and interdisciplinary approaches
Summary: Animal models have greatly facilitated the process of drug discovery. Len Zon draws on his own experiences to highlight how a cross-model, interdisciplinary approach can bring basic findings to the clinic.
- Cellular dynamics of regeneration reveals role of two distinct Pax7 stem cell populations in larval zebrafish muscle repair
Summary: Tracking muscle precursor cells during wound repair with confocal time-lapse microscopy reveals that two myoblast populations fuse and contribute differentially to regeneration, one initiating and the other growing fibres.
- Muscles provide protection during microbial infection by activating innate immune response pathways in Drosophila and zebrafish
Summary: Using fruit fly and zebrafish models, we show that skeletal muscles are immune responsive tissues; they mount innate immune responses during bacterial infection – an evolutionarily conserved defense mechanism.